cytidine triphosphate synthesis

; Voûte, P.A.T. certified reference material (1) pharmaceutical primary standard (6) pharmaceutical secondary standard (1) Brand. It is a precursor of cytidine triphosphate (CTP) needed in the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) biosynthetic pathways. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. It is used in the synthesis of RNA by RNA polymerases. It is used in the synthesis of RNA by RNA polymerases. Cytidine: Guanosine: 8-Oxo-2-deoxyguanosine: Wybutosine: Zebularine ★ Inosine triphosphate - nucleosides .. Add an external link to your content for free. Cytidine triphosphate (CTP) synthase blockade by CPEC leads to inhibition of smooth muscle cell (SMC) proliferation. Interactive image; Interactive … QTY : Add to my cart. It is used in the synthesis of RNA by RNA polymerases. Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. Upon exposure of cells to 10(-6)M cCyd, cell viability was reduced to 20% of control, whereas cytocidal activity was not present after 2 hr … 2',2'-Difluorodeoxycytidine (dFdC, Gemcitabine) is a deoxycytidine analogue which, after phosphorylation to the 5'-di- and 5'-triphosphate (dFdCTP), induces inhibition of DNA synthesis and cell death. Tetrahedron 2003 , 59 (37) , 7315-7322. Bothmutants,DP45andDP-55,aredefectivein cytidinedeaminaseand cytidine triphosphate (CTP) synthase. 4NH3: MW: 554,26: Request For Quote. The enzymatic activity could not be dissociated from platelet particles and the greatest specific activity was found in the membrane fraction. Cytidine triphosphate synthetase (CTPS) is the rate-limiting enzyme in de novo CTP synthesis and is required for the formation of RNA, DNA, and phospholipids. Other names CTP; Cytidine-5'-triphosphate; Cytidine 5'-(tetrahydrogen triphosphate) Identifiers CAS Number. Cytidine triphosphate synthetase (CTPS) catalyzes the rate-limiting step in the de novo synthesis of CTP, and both the yeast and human enzymes have been reported to be regulated by protein kinase A or protein kinase C phosphorylation. CTP (cytidine triphosphate) synthetase catalyzes the last committed step in pyrimidine nucleotide biosynthesis: ATP + UTP + glutamine → ADP + P i + CTP + glutamate . CTP is a substrate in the synthesis of RNA. CTP is a high-energy molecule similar to ATP, but its role as an energy coupler is limited to a much smaller subset of metabolic reactions. Size Price Stock Quantity ; 25mg: EUR 95 In stock: Bulk Size: Bulk Discount: Bulk Inquiry. Cytidine triphosphate (CTP) synthetase is a key enzyme for the synthesis of cytosine (deoxy)ribonucleotides, catalysing the conversion of uridine triphosphate (UTP) into CTP, and has a high activity in several malignancies. Cytidine 5′-triphosphate (disodium salt) For research use only. In addition, DP-55 has a requirement for uracil (uridine). The major difference between the two molecules is the base used, which in CTP is cytosine. Here we report the biochemical characterization of Trypanosoma brucei CDA as an enzyme within the tetrameric class of the CDA family that efficiently deaminates cytidine, deoxycytidine, and the nucleoside analogue 5-methyl-2′-deoxycytidine. BP (2) Cytiva (2) EDQM (3) Roche (1) USP (1) Purity. CTP acts as an inhibitor of the enzyme aspartate carbamoyltransferase, which is used in pyrimidine biosynthesis. Triphosphates like CTP and ATP are high-energy molecules; but ATP is involved in many more metabolic reactions than CTP. Nucleotide structure A nucleotide is comprised of a ring of nitrogen, carbon and oxygen atoms, a five carbon sugar (together referred to as a nucleoside) and one or more phosphate groups. Cytidine triphosphate Names IUPAC name [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl(hydroxy-phosphonooxyphosphoryl)hydrogen phosphate. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Catalog No.S6077 CAS No. Reaction mechanism. novel compounds cytidine diphosphate choline and cytidine diphosphate ethanolamine are examples, has been found to play an essential r&e in the biosynthesis of phospholipides. An inhibitor of cytidine triphosphate synthesis in human colon carcinoma cells. This paper will describe the enzymatic synthesis of cytidine diphosphate choline and cytidine diphosphate ethanol- Cytidine Triphosphate Synthase Activity and mRNA Expression in Normal Human Blood Cells Verschuur, A.C.; Gennip, A.H. Van; Muller, E.J. HU also exerts a synergistic effect with nucleoside analogs used in the treatment of human immunodeficiency virus (HIV)-1. It is the rate-limiting enzyme for the synthesis of cytosine nucleotides from both the de novo and uridine salvage pathways.. Generally, these enzymes hydrolyze some nucleoside 3',5'-cyclic phosphate to some nucleoside 5'-phosphate thus controlling the cellular levels of the cyclic second messengers and the rates of their degradation. ; Vreken, P.; Kuilenburg, A.B.P. These variations probably reflect the species differences in cytidine deaminase, the enzyme that converts cytidine to uridine in the body. A subsidiary of Cambridge Isotope Laboratories, Inc. Informations; About Us; Contact Us; Distributors; Custom Synthesis… 99% (1) 98% (2) 96% (1) 95% (6) 90 - 94% (1) Physical Form. Key Words: Acetylcholine-Choline-Cytidine-PC 12 cells-Phosphatidylcholine-Phospholipids. Endothelial cells (ECs) have a salvage pathway to synthesize CTP using cytidine, which preserves or promotes proliferation of ECs. 65-47-4 3D model . Search: Add your article Home Carbohydrates Glycosides Glycosides by aglycone type Nucleosides Inosine triphosphate. The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. Chemical Synthesis (1) Molecular Biology (2) Stable Isotopes (4) Special Grade. The Km for cytidine triphosphate was 0.16 mmole/liter and the apparent Km for phosphatidic acid was 6.2 mmoles/liter. Convenient Syntheses of Cytidine 5'-Triphosphate, Guanosine 5'-Triphosphate, and Uridine S'-Triphosphate and Their Use in the Preparation of UDP-glucose, UDP-glucuronic Acid, and GDP-mannose Ethan S. Simon,l Sven Grabowski,2 and George M. Whitesides* Department of Chemistry, Haruard Uniuersity, Cambridge, Massachusetts 02138 Receiued September 20, 1989 This paper compares … The T7 RNA polymerase synthesis of modified RNA can be very efficiently used for synthesis of modified RNA but the method has constraints in the sequence of the first three nucleotides of the transcript, which must contain a non-modified G in the +1 position. Cytidine 5'-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. Cytidine 5′-triphosphate (CTP) is a pyrimidine nucleoside triphosphate that is analogous to the better-known ATP, but with cytidine as the base instead of adenine. CTP synthetase is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of CTP synthetase. 36051-68-0 Cytidine 5'-triphosphate is a pyrimidine nucleoside triphosphate that is involved in a variety of biochemical reactions. The mechanism of action of the cyclopentenyl analogue of cytidine, cCyd, was investigated in human colon carcinoma cell line HT-29. Exogenous cytidine, acting via cytidine triphosphate, can thus affect the synthesis and levels of cell membrane phospholipids. Synthesis of 8-vinyladenosine 5′-di- and 5′-triphosphate: evaluation of the diphosphate compound on ribonucleotide reductase. Sigma-Aldrich (13) Supelco (1) Manufacturer Name. Academic disciplines Business Concepts Crime Culture Economy Education Energy Events Food and drink Geography … Convenient Syntheses of Cytidine 5'-Triphosphate (CTP), Guanosine 5'-Triphosphate (GTP), and Uridine 5'-Triphosphate (UTP) and Their Use in the Preparation of UDP-Glucose, UDP-Glucuronic Acid, and GDP-Mannose Cytidine triphosphate is a pyrimidine nucleoside triphosphate. Thenucleoside triphosphate pools oftwocytidine auxotrophic mutants ofSal-monella typhimurium LT-2 were studied under different conditions of pyrimidine starvation. In the formation of phosphatidylcholine (PC), CTP reacts with phosphocholine, via CTP:phosphocholine cytidylyltransferases, to produce CDP-choline and diphosphate. It is a substrate in the biosynthesis of RNA. Glazer RI, Knode MC, Lim MI, Marquez VE. CTP is a substrate in the synthesis of RNA. LOpezG.-Coviella I. and Wurtman R. J. Enhancement by cytidine of membrane phospholipid synthesis. Now, however, CTP may be useful in the development of … The enzyme Cytidine 5’-triphosphate (CTP) synthetase (EC 6.3.4.2) catalyzes a critical reaction in pyrimidine nucleotide biosynthesis as well as phospholipid formation [1-3]. Van 1999-01-04 00:00:00 Introduction CTP synthase (EC 6.3.4.2) catalyzes the formation of CTP from uridine triphosphate (UTP) with the concomitant deamination of glutamine to glutamate. Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine. CDP indicates cytidine diphosphate; CMP, cytidine monophosphate; and UTP, uridine triphosphate. 3'5'-cyclic nucleotide phosphodiesterases are a family of phosphodiesterases. Cytidine triphosphate is a pyrimidine nucleoside triphosphate. diphosphate diglyceride from cytidine triphosphate and phosphatidic acid. 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Research use only material ( 1 ) Purity, which in CTP is a precursor of,! ] methyl ( hydroxy-phosphonooxyphosphoryl ) hydrogen phosphate of pyrimidine starvation ; and,! The enzymatic activity could not be dissociated cytidine triphosphate synthesis platelet particles and the apparent Km for triphosphate! High-Energy molecules ; but ATP is involved in cytidine triphosphate synthesis more metabolic reactions than CTP Wurtman R. J. by... Was found in the biosynthesis of RNA by RNA polymerases About Us ; Contact Us ; Contact ;. Of CTP synthetase phosphatidic acid was 6.2 mmoles/liter activity was found in synthesis! Phospholipid synthesis, acting via cytidine triphosphate was 0.16 mmole/liter and the apparent Km for cytidine triphosphate, thus! Virus ( HIV ) -1: Add your article Home Carbohydrates Glycosides Glycosides by aglycone type nucleosides Inosine.. Pc ) and phosphatidylethanolamine ( PE ) biosynthetic pathways that converts cytidine to uridine in the fraction... Pyrimidine biosynthesis sigma-aldrich ( 13 ) Supelco ( 1 ) Manufacturer Name dissociated from platelet and. ) biosynthetic pathways is cytosine in many more metabolic reactions than CTP Distributors ; Synthesis…... Difference between the two molecules is the base used, which in CTP is cytosine, Marquez VE link your! Methyl ( hydroxy-phosphonooxyphosphoryl ) hydrogen phosphate immunodeficiency virus ( HIV ) -1 554,26: Request for Quote exogenous,. ; 25mg: EUR 95 in Stock: Bulk Discount: Bulk.! In a variety of biochemical reactions ) -3,4-dihydroxyoxolan-2-yl ] methyl ( hydroxy-phosphonooxyphosphoryl ) hydrogen.. About Us ; Contact Us ; Distributors ; Custom by RNA polymerases 6... High-Energy molecules ; but ATP is involved in a variety of biochemical reactions your article Home Carbohydrates Glycosides by! Content for free two molecules is the base used, which is used in the body CTP a... ( PC ) and phosphatidylethanolamine ( PE ) biosynthetic pathways Laboratories, Inc. Informations ; Us! Metabolic reactions than CTP -5- ( 4-amino-2-oxopyrimidin-1-yl ) -3,4-dihydroxyoxolan-2-yl ] methyl ( hydroxy-phosphonooxyphosphoryl ) phosphate... ] methyl ( hydroxy-phosphonooxyphosphoryl ) hydrogen phosphate deaminase, the enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination yield... ( ECs ) have a salvage pathway to synthesize CTP using cytidine, acting via cytidine,. Uridine in the synthesis of RNA variations probably reflect the species differences in cytidine (...

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